Towards Fieldable Rapid Bioagent Detection: advanced Resonant Optical Waveguide and Biolayer Structures for Integrated Biosensing
Abstract
This research project established the understanding necessary for the stacked planar affinity-regulated resonate optical waveguide (SPARROW) architecture to serve as a transducer device platform for rapid biomolecular detection. SPARROW structures have been grown with waveguide losses of less than 1 dB/cm. Thin biolayers of less than 10 nm were achieved and SAM attachment to polycrystalline surfaces studied. SPARROW transducer measurements were conducted using sucrose analyte solutions applied to the top waveguide surface via a PDMS microfluidic channel. For a flow channel and waveguide interaction width of 1200 microns, detection equivalent to a surface loading of from 10 to 1 pg/mm^2 was achieved under different SPARROW surface conditions. Achievable detection was modeled and indicated a Limit of Detection (LODs) lower than 0.1 pg/mm^2 for spore sized targets.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2007
- Accession Number
- ADA473931
Entities
People
- Aaron Timperman
- Dimitris Korakakis
- Lawrence A. Hornak
Organizations
- West Virginia University