Development of Micro-Scale Assays of Mammary Stem and Progenitor Cells

Abstract

The main focus of this work is to study the effects of population demographics in the mammary gland on cancer risk using mathematical means and employ microtechnology for in vitro studies of primary cell characteristics. Specific attention has been paid to developing more quantitative methods for analyzing microfluidic cell cultures using In Channel Westerns. Also understanding how the microfluidic culture platform differs from traditional macro-scale techniques is critical. By thoroughly understanding how this culture platform affects the cellular baseline first better and more efficient data collection can be performed thus requiring fewer primary cells. Preliminary results showing the effectiveness and ease of using In Channel Western protocols for studying protein expression in microfluidic cultures is promising.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2007
Accession Number
ADA474019

Entities

People

  • Amy L. Paguirigan

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Biomedical Engineering
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Demography
  • Electronic Mail
  • Engineering
  • Glands
  • Growth Factors
  • Mammary Glands
  • Mathematical Models
  • Neoplasms
  • Platforms
  • Proteins
  • Stem Cells

Readers

  • Integrated Circuit Design and Technology.
  • Molecular Biology and Genetics
  • Systems Analysis and Design