Three Minutes-Long Electrophoretically Assisted Zeptomolar Microfluidic Immunoassay with Magnetic-Beads Detection
Abstract
An ultrasensitive two-step immunoassay was developed for rapid assay of proteins and viruses. In the first step, electrophoresis was used to quickly bring soluble analytes from a flowing solution to a microarray of probe molecules immobilized on s semi-permeable membrane. in the second step, the captured analyte was detected by scanning the mcroarray with functionalized magnetic beads passed over the array surface by shear flow and pressed to the array surface by a magnetic field.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 24, 2007
- Accession Number
- ADA474648
Entities
People
- C. Bailey
- Michael J. Turell
- S. Groves
- V. N. Morozov
Organizations
- United States Army Medical Research Institute of Infectious Diseases