A Targeted Multifunctional Platform for Imaging and Treatment of Breast Cancer and Its Metastases Based on Adenoviral Vectors and Magnetic Nanoparticles
Abstract
Nanotechnology holds many promises for the imaging and treatment of breast cancer. In particular, magnetic nanoparticles can be utilized for tumor imaging via magnetic resonance imaging (MRI) techniques and tumor treatment by heating after exposure to an alternating magnetic field. However, selective targeting of the nanoparticles to the tumor cells needs to be accomplished before the imaging or therapy can be successful. In this respect, adenoviral (Ad) vectors for gene therapy have made great progress in achieving successful tumor targeting after their intravenous administration. We therefore hypothesized that coupling magnetic nanoparticles to targeted Ad vectors would allow the selective localization of these particles to the tumor, thereby making imaging and therapy possible. We herein identified that coupling the nanoparticles to the Ad capsid protein hexon does not perturb vector infectivity or retargeting efficiency in vitro. Furthermore, we have demonstrated the intracellular accumulation of nanoparticles targeted to breast cancer cells using this system. We will continue this project by identifying novel magnetic nanoparticles with optimal magnetic contrast properties for imaging and treatment of breast cancer cells, and will also broaden our project's scope by including Quantum Dots in addition to magnetic nanoparticles for the imaging of tumors. This will further our goal to develop multifunctional systems for targeting, imaging and treatment of cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2007
- Accession Number
- ADA474672
Entities
People
- Maaike Everts
- Vaibhav Saini
Organizations
- University of Alabama