Improving Breast Cancer Diagnosis by Antisense Targeting

Abstract

The goal of this investigation is to using anti-Her2 antibody Herceptin as carrier and streptavidin as linker to specifically transport antisense into tumor cells expressing Her2. For this purpose we successfully conjugated MAG3/biotin to antisense/sense morpholino oligomers (MORFs) and conjugated biotin group to Herceptin. The MORF-streptavid in-Herceptin constructs have been synthesized and their quality has been confirmed. We have confirmed by confocal microscopy using fluorescent lissamine as the tag that Herceptin can mediate the cellular internalization of MORFs and more important the internalized oligomer distributed in cytoplasm evenly without apparent entrapment in cellular compartments. By using 99mTc as the tag we also have approved that the antisense can be released from the internalized antisense-streptavidin-Herceptin construct. These results are significant and encouraging for the followed studies in in vivo antisense tumor targeting using Herceptin as carrier.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2007
Accession Number
ADA474678

Entities

People

  • Yi Wang

Organizations

  • University of Massachusetts Medical School

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antibodies
  • Antisense Elements (Genetics)
  • Bacterial Proteins
  • Breast Cancer
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Confocal Microscopy
  • Detection
  • Ethers
  • Gene Expression
  • Molecules
  • Nucleic Acids
  • Oligomers
  • Peptides
  • Proteins
  • Tumor Cell Line

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular and Cellular Biochemistry