Molecular Genetic Studies of Bone Mechanical Strain and of Pedigrees with Very High Bone Density
Abstract
The primary goal of the proposed work on bone mechanical strain focuses on identifying the genes and their functions involved in mediating the anabolic skeletal response to mechanical stress. Two hypotheses have been proposed: I) Quantitative trait loci analysis using the four point bending technique in two strains of mice exhibiting extreme differences in loading response will lead to identification of chromosomal locations of genes involved in variation in skeletal response to mechanical loading. 2) Application of microarray and tyrosine phosphorylation studies using bone cells derived from inbred strains of mice exhibiting extreme differences to loading response and physiologically relevant fluid flow shear strain will lead to identification of key signaling genes and their pathways that contribute to variation in bone cell response to mechanical strain. During the last funding period we proposed several specific objectives for each of the above-mentioned hypothesis. We have made considerable progress in accomplishing all of the specific objectives. Our work during this reporting period has resulted in one published manuscript and two abstracts. We believe that successful accomplishment of the proposed studies will provide a better understanding of the molecular mechanisms involved in identifying the genes and their function as related to mechanical stress.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2007
- Accession Number
- ADA475118
Entities
People
- Subburaman Mohan