Identify the Impact of TGF-Beta Signaling on the Stroma in the Progression of Prostate Cancer
Abstract
As a result of androgen ablation TGF- 1 expression levels transiently elevate and regression of benign prostate hyperplasia as well asprostate cancer cells for the most part occur. Better understanding of prostate androgen responsiveness is critical in understanding and ultimately combating androgen-non-responsive prostate cancer. Studying the conditional TGF- type II receptor fibroblast knock out mouse model we developed (Tgfbr2fspko), we found that TGF- signaling in the prostate stromal fibroblasts regulate both stromal and epithelial differentiation in the prostate. Notably the data dispels previous reports that TGF- signaling is required for myofibroblast differentiation. As proposed we attempted to develop mice that are stromally knocked out for TGF- signaling and express the large Tantigen in the prostate epithelia, but was unsuccessful. We have however acquired techniques in our laboratory to perform tissue recombination experiments where the identical cell types (prostate stroma and epithelia) can generate prostate glands through xenografting, that display similar phenotypic characteristics of intact mice. We hope to gain permission to progress with these experiments in order to address the mechanism of stromal TGF- signaling impact on prostate cancer androgen responsiveness.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2007
- Accession Number
- ADA475121
Entities
People
- Neil A. Bhowmick
Organizations
- Vanderbilt University