A Chemical Strategy to Trap and Identify Proteins That May Regulate Promoter Hypermethylation in Breast Cancer
Abstract
With the support from this grant we have been developing photocross-linking DNA base analogues to trap and identify DNMTs and interacting proteins on specific promoter DNA. To this end Diazirine photophore was introduced into either the major or minor groove of DNA as a photocross linking group via a convertible nucleoside methodology. The resulting DNA probes efficiently cross linked with the E. coli DNA adenine methyltransferase (EcoDam). The diazirine photophore possesses clear advantages over other photocross linking groups previously installed on DNA in that it has the least steric hindrance and efficiently generates a carbene intermediate that non-specifically cross links with protein residues nearby in high efficiency. We have also applied the method to human cell extracts and obtained very promising results. Based on this early success we are working on the BRCA1 promoter DNA and hope to identify the DNMT that works on this promoter in the near future.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2007
- Accession Number
- ADA475123
Entities
People
- Chuan He
Organizations
- University of Chicago