Pilot Comparison of Stromal Gene Expression Among Normal Prostate Tissues and Primary Prostate Cancer Tissues in White and Black Men
Abstract
This hypothesis development project tested the feasibility of identification, laser capture microdissection, and expression analysis of prostate-stroma specific cells in normal and cancerous prostates, and aims to develop preliminary data sufficient to identify potential differences in stromal RNA expression in normal and cancerous prostate tissue. Our studies found that it is difficult but not impossible to histologically identify prostate zones with an acceptable degree of confidence in frozen tissues, eliminating the need to attempt expression studies in fixed tissues with their attendant biases. LCM of stromal tissue was completed for 6 normal prostates from men across the age range and of the two racial groups studied, and from prostates from men of similar ages with adenocarcinoma identified distant from the area of dissection. High Quality RNA was isolated, and duplicate Affymetrix Plus 2.0 chip analysis performed. We observe potentially significant expression differences in both normal epithelial and stromal cells from fully normal prostates as compared to prostates containing adenocarcinoma which are now subject to formal hypothesis testing.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2007
- Accession Number
- ADA475126
Entities
People
- G. S. Bova
Organizations
- Johns Hopkins University