Sam68 and Breast Cancer

Abstract

Sam68 is a target for Src kinase. It is involved in HIV-1 RNA export. It has been well documented that patients have either lost or have mutations in BRCA1 or BRCA2 tumor suppressor genes are at high risk of developing breast cancers. Thus, the major goal of this application was to investigate whether Sam68 is involved in the export of BRCA1 mRNA. The other goal was to map the BRCA1 mRNA export pathway. To test this objective, we knocked down Sam68 in MCF7 cells. Sam68 knockdown cells expressed reduced levels of BRCA1 mRNA. However, it is not known whether the effect on BRCA1 is due to the transcriptional repression or RNA stability. More evidence came from the Sam68 dominant negative mutant approach, in which BRCA1 protein is repressed. This may account for the RNA destability. Using the Leptomycin B, we have shown that BRCA1 mRNA uses CRM1-dependent pathway. Taken together, our results suggest that Sam68 regulates BRCA1 RNA metabolism.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2007
Accession Number
ADA475146

Entities

People

  • Thipparthi R. Reddy

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Carrier Proteins
  • Cell Line
  • Cells
  • Mammary Glands
  • Metabolism
  • Mrna
  • Mutations
  • Neoplasms
  • Pcr Testing
  • Proteins
  • Rna Stability
  • Suppressors
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Electrochemical Surface Science
  • Molecular Biology and Genetics