Molecular Mechanisms of Nonlinearity in Response to Low Dose Ionizing Radiation
Abstract
In this very new project we have begun a systematic evaluation of the molecular mechanisms of radiation adaptation. We have developed a wound-healing model that will provide a functional assessment of the promitogenic effects of low dose radiation exposure. We have begun detailed cell cycle analysis of low dose radiation exposure on human keratinocytes and fibroblasts as well as survival as says following priming and challenge doses of ionizing radiation. Initial experiments suggest that doses in the range of 10 cGy provide a promitogenic signal. Further, a priming dose of 10-20 cGy seems to provide radiation protection against subsequent challenge doses up to 4 Gy. Beyond 4 Gy in challenge doses, the radioprotective effect of the priming dose seems to be overwhelmed. Genomic evaluation using the Illumina microarray platform is ongoing.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 12, 2007
- Accession Number
- ADA475210
Entities
People
- David M. Rocke
- Zelanna Goldberg
Organizations
- University of California