Evaluation, Validation, and Demonstration of a Total Protein Assay for Application to Biotoxin Fate Studies

Abstract

This report summarizes efforts to evaluate, optimize, and validate a commercially available analytical assay for the quantitative determination of total protein in aqueous sample matrices. The data generated during this study will be used to support on-going investigations into the fate of biotoxins in aqueous environments. The optimized assay was found to be repeatable, reproducible, and not subject to interference by several different water matrices. The method limit of detection was determined to be 0.350 mg/L, and the method limit of quantization was determined to be 1.17 mg/L. The linear range was determined to be 1.00 to 25.0 mg/L of total protein.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2007
Accession Number
ADA475212

Entities

People

  • Amanda M. Schenning
  • Jeffrey S. Rice
  • Kevin M. Morrissey
  • Vicky L. Bevilacqua

Organizations

  • Leidos

Tags

Communities of Interest

  • Counter WMD

DTIC Thesaurus Topics

  • Accuracy
  • Albumins
  • Amino Acids
  • Biological Toxins
  • Buffers (Chemistry)
  • Chemical Synthesis
  • Chemistry
  • Demonstrations
  • Detection
  • Environment
  • Measurement
  • Reaction Time
  • Sodium
  • Sodium Azides
  • Sodium Compounds
  • Sodium Hydroxide
  • Test And Evaluation

Readers

  • Groundwater Contamination Remediation.
  • Microbial Pathology
  • Systems Analysis and Design