Identification of Serine Proteinases Involved in Breast Cancer Progression
Abstract
The objective of the grant was to identify serine proteinases (a) upregulated in malignant breast epithelial T4-2 cells, and (b) for which specific inhibition by RNA interference (RNAi) results in suppression of the malignant phenotype, as assessed in three-dimensional culture assays. We successfully identified five serine proteinases significantly upregulated in T4-2 cells: mesotrypsin, prostasin, transmembrane protease serine 3 (TMPRSS3), neurotrypsin, and matriptase-1. Among these, inhibition of mesotrypsin expression in T4-2 cells by RNAi leads to a significant reduction in colony size in 3D culture, implicating mesotrypsin activity in the T4-2 cell malignant growth phenotype, and identifying mesotrypsin as an oncogenic serine proteinase and a potential therapeutic target.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2007
- Accession Number
- ADA475755
Entities
People
- Evette S. Radisky
Organizations
- Mayo Clinic