Addressable Immobilized Ion Channels: Optimization of Ion Channels Tethered to Device Surfaces
Abstract
This work is performed by a team at Florida, Mainz, Texas, Agave Biosystems and Miami. We are attempting to develop a sensitive and selective biomolecular detection system based on arrays ion channel covalently tethered over microelectronic devices. The technical approach involves inserting ion channels in non-native membrane bilayers, characterizing and optimizing the single channel stochastic response, and tethering these assemblies over device gate surfaces. Enhancing the stability of the assemblies is another important part of the program. The sensing will be based on this device architectures whereby the placement of individual analyte molecules at the throat of the ion channel will in turn change the stochastic signature associated with the ion translocation measured by the device. Optimizing and tethering the channels, enhancing stability, and demonstrating chem/bio agent signatures as well as interferants. There have been a number several recent accomplishments in this program. We have obtained gigaohm seals and formed these seals on gold micro electrodes with >80% pixel-to-pixel seal formation. We have measured single channel activity from more than ten different ion channel systems including three genetic variants of Maxi-K, Uranacidin A, and a mechanosensitive channel. We obtained on-device pharmacological response from micromolar organophosphate and could wash it off the device. We assembled S-layer proteins at the lipid bilayer interface, enhancing stability. We studied bilayer formation by QCN-d.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 25, 2007
- Accession Number
- ADA476025
Entities
People
- Randy Duran
Organizations
- University of Florida