Dysregulation of RNA Interference in Breast Cancer
Abstract
The newly discovered RNA interference is a novel type of gene regulation mechanism, which is required for normal expression of genes. This study tests the hypothesis that breast tumor carries dysregulated RNA interference pathways, and thus, some tumor suppressor genes will be down-regulated while other genes (e.g., oncogenes) will be up-regulated, leading to tumor cell proliferation and survival. Using real time RT-PCR, we demonstrate that microRNA-21 is overexpressed in breast tumors compared to the matched normal breast tissue. Furthermore, we show that antisense oligonucleotide against microRNA-21 can suppress the endogenous microRNA-21 and causes tumor cell growth inhibition. Experiments with a xenograft carcinoma mouse model reveal that the antisense microRNA-21 oligonucleotide also inhibits tumor growth. Therefore, microRNA-21 is a potential therapeutic target for breast cancer therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2007
- Accession Number
- ADA476753
Entities
People
- Yi-yuan Mo
Organizations
- Southern Illinois University