PSMA-Targeted Nano-Conjugates as Dual-Modality (MRI/PET) Imaging Probes for the Non-Invasive Detection of Prostate Cancer
Abstract
The goal of this project is to develop dual modality imaging probes for the detection of prostate cancer by doping radioisotopes to iron oxide nanopartides so that the sensitivity and specificity of prostate cancer diagnosis could be significantly improved. In the second year a rigorous synthetic protocol has been developed to prepare dextran TI 0-coated iron oxide nanoparticles with uniform size distribution and better controllable reproducibility compared to the method developed in the first year. Protocol of conjugating nanoparticles with prostate cancer targeting molecules has been successfully established. Two nanoparticles with mean sizes of 11=8 and 30.6 nm (radii) were evaluated in vitro and in vivo. The relaxivity values (r2) of the prepared nanoparticles were observed up to 43.3 mM-Is-I with the r21rI ratio being 88.4 exhibiting potential application of contrast enhancement on T2- and T2*-weighted MR images. Both nanoparticles showed excellent in vitro stability in rat serum. The biodistribution studies in normal animal showed that the smaller nanoparticle (NP-I radius 11.8 nm) has a better tissue distribution profile than the larger one (NP-2 radius 30.6 nm). Impressively NP-I showed remarkable tumor uptake in a PC-3 xenograft model with a tumor to muscle ratio of 12.11 i 3.87 at 24 h post injection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2007
- Accession Number
- ADA476783
Entities
People
- Xiankai Sun
Organizations
- University of Texas at Dallas