The Transgenic TGF-Alpha or EGFR1 Overexpression Mouse Model for Symptom Complex Research
Abstract
The TGF- transgenic over expression model has been used to study circadian running wheel activity (RWA) in C57Bl6 mice. The genotype of each transgenic animal is determined by PCR analysis of tissue and gel electorphoresis. Male and female transgenic and control animals have been assessed for RWA, phase, and total amount of activity in a light controlled environment using a 12:12 hour light/dark (LD) cycle and in constant darkness (DD). The results show an intact central clock in LD and DD condition in each gender when assessed for phase of the circadian period. The endogenous rhythm of both the transgenic and wild type animals is 23.45 and 23.55 hours for female and males animals, respectively. The measure of the relative duration of activity in the transgenic animals is significantly shorter compared to controls and is consistent with our hypothesis that overproduction of TGF- is associated with inhibition of activity. Our next phase of study will use this established model to test the effect of a tyrosine kinase inhibitor on activity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2007
- Accession Number
- ADA477134
Entities
People
- Tyvin Rich
Organizations
- University of Virginia