VEGF-Iron Oxide Conjugate for Dual MR and PET Imaging of Breast Cancer Angiogenesis

Abstract

The overall hypothesis of this concept award proposal is that a single probe that allows simultaneous acquisition of both positron emission tomography (PET) and molecular magnetic resonance imaging (mMRI) data will maximizes the topographical, anatomical, functional, and molecular information of breast cancer. In this proposal, we choose to image breast cancer tumor vasculature by visualization and quantification of Flt-1 and Flk-1/KDR. Objectives: Aim 1: To develop iron oxide (IO) nanoparticles functionalized with both VEGF121 and PET isotope 64Cu (t1/2 = 12.7 h) and test the dual probe in vitro. Aim 2: To test the PET and mMRI efficacy of the dual-functional nanoprobe in breast cancer in vivo. Major Findings: We have developed VEGF121 mutant that is VEGFR-2 specific. We have developed water soluble iron oxide nanoparticles conjugated with macrocyclic chelating agent DOTA for 64Cu-labeling and cyclic RGD peptide for integrin alpha(v)beta(3) recognition. We have also developed IO-VEGF conjugate and tested the nanoconstruct.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2007
Accession Number
ADA477283

Entities

People

  • Xiaoyuan Chen

Organizations

  • Stanford University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acquisition
  • Breast Cancer
  • Chelate Compounds
  • Cyanides
  • Detection
  • Integrins
  • Iron
  • Iron Oxides
  • Metal Oxide Nanoparticles
  • Nanoparticles
  • Neoplasms
  • Oxides
  • Particles
  • Positron Emission Tomography
  • Positron Emissions
  • Positrons
  • Tomography

Fields of Study

  • Medicine
  • Physics

Readers

  • Medical Imaging.
  • Oncology (Cancer Research).
  • Organic Chemistry

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech