Genetic Alterations in Epithelial and Stromal Compartments of Prostate Adenocarcinomas
Abstract
Genetic analyses on prostate cancer has been occurring for over a decade. However such studies were uniformly performed on the entire tumor without regard to its components despite the fact that a few groups were quite aware of both epithelial and stromal components of tumors and the cell biology of the tumor "microenvironment" has been described for the last 20 years. Thus until now when a genetic alterabon be it intragenic mutabon regional amplificabon or deletion manifested by loss of heterozygosity of markers (LOH) is attributed to a prostate cancer it is unclear if the alteration is actually occurring in the epithelial compartment the surrounding stromal compartment or both. Our own preliminary data on breast carcinomas demonstrate that LOH and even somatic mutations can occur in surrounding stromal fibroblasts. Therefdre this proposal proposes to search for genetic alterations in the stroma of prostate cancers and to determine if such alterations can influence clinical outcome. In the first year we have accrued 55 distinct non-M1 sporadic adenocarcinomas of the prostate have subjected them to laser capture microdissection to separate compartments and are about to begin a total genome LOH scan.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2006
- Accession Number
- ADA477380
Entities
People
- Charis Eng
Organizations
- Ohio State University