A New Concept for Androgen Receptor-Independent Growth of Prostate Cancer

Abstract

Angiogenin is progressively upregulated in prostate cancer, in particular in androgen-independent diseases. The objective of this project is to explore the role angiogenin plays in the development of androgen-independent disease. In this reporting period, we have demonstrated that nuclear translocation of angiogenin is specific for prostate cancer cells and does not occur in normal prostate epithelial cells. Angiogenin is translocated to the nucleus of androgen-dependent cells (LNCaP) only when the cells are stimulated with androgen (DHT). But angiogenin is constitutively translocated to the nucleus of androgen-independent cells (PC-3, PC-3M, DU145). Angiogenin is required for DHT to stimulate rRNA transcription and cell proliferation of androgen-dependent cells. Overexpression of angiogenin enables androgen-independent growth of otherwise androgen-dependent prostate cancer cells in vitro and in vivo. Finally, we have shown that angiogenin binds to the promoter region of rDNA in vivo and stimulates rRNA transcription.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2007
Accession Number
ADA477419

Entities

People

  • Guo-fu Hu
  • Hiroko Kishikawa
  • Norie Yoshioka

Organizations

  • Harvard Medical School

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Androgen Receptors
  • Androgens
  • Antibodies
  • Biomedical Research
  • Cancer
  • Cells
  • Chemistry
  • Diseases And Disorders
  • Electronic Mail
  • Endothelial Cells
  • Epithelial Cells
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Proteins
  • Transcription Factors

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Prostate Cancer Biology.