Amplification of Type II Cadherins in Prostate Cancer
Abstract
Genomic alterations of 18q have been observed in prostate cancer. This research focuses on analyzing the role of increased gene copy number at 18q22.1 in prostate cancer. We believe the key genes in this region are type II cadherins. We are studying the role of overexpression of cadherin 7 (CDH7) on the tumorigenic and invasive potential of prostate cancer cells. The increased copy number of CDH7 is specific to prostate cancer and results in increased levels of CDH7 mRNA in prostate tumors. We previously created and purified polyclonal antibodies against CDH7 peptides to use in immunohistochemistry experiments to determine if increased CDH7 copies increases the protein level, but these antibodies proved to be inadequate. We have since created polyclonal and monoclonal antibodies against full-length bacterially-expressed protein and are in the process of testing these antibodies. We have already performed knockdown experiments of the CDH7 mRNA in a prostate cancer cell line and will analyze the CDH7 protein levels in these cells with these new antibodies. We will subsequently evaluate these knockdown cells for invasive and tumorigenic potential.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2007
- Accession Number
- ADA477469
Entities
People
- Teresa L Johnson-Pais
Organizations
- University of Texas at San Antonio