Temporal and Spatial Dynamics of Androgen Receptor Conformation and Interactions in Prostate Cancer Cells
Abstract
Our long-term goal is to correlate the undesired escape from androgen deprivation therapy with specific molecular events in Androgen Receptor signaling in order to determine the best molecular targets for prostate cancer treatment. Studies supported by this grant indicate that the failure of tumors to respond to anti-androgen therapy corresponded best with an increased nuclear transport of AR. However, an intramolecular fold and AR dimerization, both activated abnormally by heterologous hormones (estrogen and progestin) and measured by fluorescence resonance energy transfer of CFP and YFP-tagged ARs), also was linked to four different AR mutants associated with treatment-refractory prostate proliferation. High throughput methods were developed to measure AR folding, dimerization and nuclear transport. These methods will facilitate the future identification of new drugs that block AR folding, dimerization and nuclear transport and that may prove useful in treatment-refractory therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2007
- Accession Number
- ADA477478
Entities
People
- Fred Schaufele
Organizations
- University of California, San Francisco