Stromal Mesenchyme Cell Genes in Prostate Cancer Development: Epigenetic Markers for Cancer and Potential Targets for Therapy
Abstract
Functional development of the prostate is governed by stromal induction and epithelial response. Given the importance of this communication it is possible that cancer could arise from a loss of this communication. We carried out comparative proteomic and transcriptome analyses to identify organ-specific stromal signaling factors from prostate and bladder stromal cells. Secreted proteins were identified by glycopeptide-capture followed by mass spectrometry. Protein quantification was done by PICA (Peptide Ion Current Area) method. A list of 116 prostate and 84 bladder glycoproteins was identified. The prostate proteins include cathepsin L. neuroendocrine convertase and tumor necrosis factor receptor involved in signal transduction extracellular matrix interaction and differentiation. For the transcriptome analysis cells were isolated by CD49a for prostate and CDI 3 for bladder. In a comparative analysis 91 bladder and 288 prostate genes were identified. Further subtractive analysis resulted in a list of 50 prostate stromal cell-specific genes. Interesting genes were CNTN1, SPOCK3 and MAOB which are known to be involved in cell interactions and organogenesis. Expression analysis showed that these genes are down-regulated in cancer suggesting their function is required in normal development.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2007
- Accession Number
- ADA478287
Entities
People
- Young A. Goo
Organizations
- University of Washington