A Genetic Approach to Define the Importance of Rheb in Tuberous Sclerosis

Abstract

This report summarizes our accomplishments in characterizing the TSC/Rheb/mTOR signaling pathway that is altered in tuberous sclerosis. We have generated mice with decreased expression of Rheb1. We have succeeded in raising an antibody against mouse Rheb2. Effects of the TSC/Rheb/mTOR signaling on cell cycle progression have been investigated and we have obtained results suggesting the involvement of p27 and AMPK. Novel activating mutations of mTOR have been identified and they were used to investigate the consequences of the activation of the TSC/Rheb/mTOR signaling pathway on cell physiology. Our study makes significant contribution to understand how the TSC/Rheb/mTOR signaling pathway is regulated. The results we obtained make important contribution to the understanding of tuberous sclerosis.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2008
Accession Number
ADA478667

Entities

People

  • Fuyuhiko Tamanoi

Organizations

  • University of California, Los Angeles

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Biomedical Research
  • Cell Cycle Proteins
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Genes
  • Inhibition
  • Kinases
  • Mutations
  • Neoplasms
  • Nutrition Disorders
  • Observation
  • Phosphorylation
  • Proteins
  • Sclerosis

Fields of Study

  • Biology

Readers

  • Aquatic Ecology
  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology