Rapid Active Assay for the Detection of Antibodies to West Nile Virus in Chickens

Abstract

To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus (WNV)-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay (commonly called a MAC-ELISA) modified for use with chicken sera.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2008
Accession Number
ADA478963

Entities

People

  • Charles L. Bailey
  • Michael J. Turell
  • Stephanie S. Groves
  • Victor N. Morozov

Organizations

  • George Mason University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical Research
  • Birds
  • Blood
  • Detection
  • Dialysis
  • Electronic Mail
  • Immunoassay
  • Infectious Diseases
  • Materials
  • Medical Personnel
  • Membranes
  • Molecules
  • Proteins
  • Sensitivity
  • Tropical Medicine
  • United States

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Virology (or Medical Virology).