Mouse Orthotopic Xenographs of Human Prostate Primary Tumors
Abstract
We implanted human prostate carcinomas either shortly after collagenase digestion or as tissue fragments obtained at surgery. We also genetically characterized tumors at harvesting and after growth in the murine host to determine if the proposed model maintains its initial genetic characteristics. After several trials in the orthotopic and subrenal capsule loci, we have implanted and begun to characterized by TMPRSS-ERG FISH and aCGH, 22 human primary prostate tumors. Results thus far show that the tumor take of prostate cancer in the subrenal capsule of mice is about 60%. Almost 80% of high grade human prostate tumors grow, whereas tumors with intermediate grade of differentiation are favored to grow if they show a high proliferation rate. By measuring serum PSA levels we can discriminate mice in which tumor xenografts grow from those in which growth of xenografts failed. Preliminary results showed that subrenal capsule xenografts maintain the same genetic background of the original parental human tumors from which they are derived. We are currently in the process of completing the genetic analysis of human tumors (with relative normals) and xenografts, to finalize our results.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2007
- Accession Number
- ADA479394
Entities
People
- Carmen Priolo
- Massimo Loda
Organizations
- Dana–Farber Cancer Institute