The Role & Action of Prohibition, an Antiproliferative Gene, in Ovarian Cancer
Abstract
The experiments proposed in this application aim at understanding the mechanisms by which prohibitin induces growth arrest in ovarian cancer. We hypothesize that the prohibitin gene product will substantially induce arrest in ovarian cancer cells and enhance differentiation. Accordingly, we will characterize the expression pattern and function of this gene. In specific aim 1, we will define the spatial, temporal and stage specific cellular expression pattern of prohibitin in normal ovary, ovarian tumors of patients with early (FIGO stage 1) and advanced (FIGO stage II-IV) stages of ovarian cancer, using immunohistochemistry, in situ hybridization, Western and Northern blot analyses. In specific aim 2, we will determine whether prohibitin over-expression results in the inhibition of ovarian cancer cell growth, using human ovarian cancer cell lines, OVCAR-3, OVCAR-8, TOV-112D, and OV-90. Lastly, in specific aim 3, we will identify and characterize the expression of variant prohibitin mRNA species in ovarian cancer. Clearly, from a clinical perspective, an understanding of prohibitin gene involvement in ovarian somatic cell growth and differentiation during the normal physiological development as well as its role in the etio-pathogenesis of ovarian tumorigenesis is important. This understanding would lead to development of novel strategies for diagnostic or prognostic therapeutics in the treatment of ovarian cancer and in the design of a more rational basis for drug development.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2007
- Accession Number
- ADA479678
Entities
People
- Winston E. Thompson
Organizations
- Morehouse School of Medicine