Role of Katanin in Prostate Cancer Bone Metastasis
Abstract
We previously identified katanin p60 as a differentially expressed protein in the bone marrow samples from prostate cancer patients with clinical evidence of bone metastasis. In order to explore the functions of katanin p60 in prostate cancer, we carried out molecular cloning and characterization of katanin p60. From prostate cancer tissues, we cloned three alternative splicing forms in addition to the full-length katanin p60. Two of isoforms showed an effect in modulating cell migration/proliferation in a wound-healing test. Meanwhile, we established stable cell lines and in vitro study systems for future studies. We will continue to characterize the functionalities of katanin p60 and isoforms by use of shRNA to down-regulate the endogenous katanin p60 in prostate cancer cells, so that to compare with those contrastingly over-expressing katanin p60 or isoform. Moreover, as planed in Statement of Work, we will conduct a correlative study of the cellular and tissue distribution of katanin p60 and isoforms in different stages of prostate cancer. Thus, the final outcome of this study will help us to understand the mechanism of katanin-mediated cell activity and to find relevant targets for cancer therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2008
- Accession Number
- ADA479752
Entities
People
- Xiang-cang Ye
Organizations
- The University of Texas MD Anderson Cancer Center