Role of Merlin in the Growth and Transformation of Arachnoidal Cells
Abstract
This proposal is concerned with the functional role of merlin in arachnoidal and meningioma cells. In year 1, we had developed meningioma-specific NF2 model systems. Now, we have finished characterizing the phenotypic effects caused by merlin loss in meningioma cell lines. We have shown that merlin loss enhances loss of contact inhibition of growth and increases anchorage independent growth. Loss of merlin also increases entry into the S-phase of the cell cycle and this effect is associated with an increase in transcript and protein levels of cyclin E1. We also find that merlin loss also decreases the apoptotic rates in meningioma cell lines. Finally, we have initiated an analysis to identify downstream targets of merlin by microarrays. In the last year of funding, we anticipate identifying and characterizing downstream effectors of merlin in meningiomas.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2008
- Accession Number
- ADA480259
Entities
People
- Anita Lal
Organizations
- University of California, San Francisco