MicroRNA and Breast Cancer Progression
Abstract
We hypothesized that certain miRNA species are differentially expressed in the normal breast epithelium and breast cancer cells. Our concept was that these miRNAs are involved in breast cancer progression by promoting either loss or alternatively an increase (in case of miRNA downregulation) in specific target mRNA translation or stability. In order to determine if certain miRNAs are involved in breast cancer we proposed to: 1. Identify the changes in miRNA expression in primary breast tumors and/or breast cancer cell lines. 2. Establish the alterations in miRNA target(s) expression or translation in breast cancer cells. Accordingly, we investigated miRNA expression in several breast cancer cell lines, including MCF10A. We consistently observed decreased expression of mir-125b and mir-145 miRNA s and increased expression of mir-21 and mir-155. Interestingly, mir-21 was progressively upregulated during oncogenic progression of MCF10A cells. In silico analysis of the potential target mRNAs include oncogenes (fos, N-myc, Fli-1) and cell cycle proteins (cdc2, cyclin D1, wee1), suggesting that miRNAs might regulate expression of these genes in breast cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2007
- Accession Number
- ADA480336
Entities
People
- Konstantin Galaktionov
Organizations
- Baylor College of Medicine