Silica-Immobilized Enzymes for Multi-Step Synthesis in Microfluidic Devices (Postprint)

Abstract

The microfluidic combinatorial synthesis of 2-aminophenoxazin-3-one (APO) is reported. Individual microfluidic chips containing metallic zinc, silica-immobilized hydroxylaminobenzene mutase and silica-immobilized soybean peroxidase are connected in series to create a chem-enzymatic system for synthesis. Zinc catalyzes the initial reduction of nitrobenzene to hydroxylaminobenzene which undergoes a biocatalytic conversion to 2-aminophenol, followed by enzymatic polymerization to 2-aminophenoxazin-3-one. Silica-immobilization of enzymes allows the rapid stabilization and integration of a biocatalyst within a microfluidic device with minimal preparation. The system proved suitable for synthesis of a complex natural product (APO) from a simple substrate (nitrobenzene) under continuous flow conditions.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2007
Accession Number
ADA481472

Entities

People

  • Bosung S. Ku
  • Heather R. Luckarift
  • Jim C. Spain
  • Jonathan S. Dordick

Organizations

  • Air Force Research Laboratory

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Air Force
  • Air Force Research Laboratories
  • Bioengineering
  • Biological Products
  • Biotechnology
  • Department Of Defense
  • Enzymes
  • Governments
  • Immobilized Enzymes
  • Information Operations
  • Military Research
  • National Governments
  • Nitrobenzenes
  • Standards
  • Synthetic Biology

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Nanofabrication and Microfabrication.
  • Neurological Diseases/Conditions/Disorders