Bacteria Classification via Surface Enhanced Raman Spectroscopy and Principal Component Analysis

Abstract

Surface-enhanced Raman scattering (SERS) provides rapid fingerprinting of biomaterial in a non-destructive manner. The adsorption of colloidal silver to biological material suppresses native biofluorescence while increasing the normal Raman signal via the surface-enhanced Raman effect. This work validates the applicability of qualitative SER spectroscopy, utilizing principal component analysis (PCA) to show discrimination of biological threat simulants, based upon multivariate statistical confidences limits bounding known data clusters. Several strains of Bacillus spores are investigated along with Pantoea agglomerans, and Brucella noetomae.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2006
Accession Number
ADA481588

Entities

People

  • A. Hyre
  • D. Emge
  • J. Guicheteau
  • L. Argue
  • S. Christesen

Organizations

  • Edgewood Chemical Biological Center

Tags

Communities of Interest

  • Counter WMD
  • Human Systems
  • Weapons Technologies

DTIC Thesaurus Topics

  • Bacteria
  • Biological Detection
  • Classification
  • Cross Correlation
  • Data Science
  • Data Sets
  • Detection
  • Factor Analysis
  • Information Processing
  • Information Science
  • Materials
  • Medical Personnel
  • Optical Properties
  • Raman Spectroscopy
  • Spectra
  • Spectroscopy
  • Statistical Analysis

Readers

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