Meeting Threat Detection Needs For The Army: Using Conjugated Polymers To Improve Diagnostics

Abstract

The focus of this work is on the development of an integrated biological detection system that can meet the needs of Army to detect biological threats. This detection system will be based on a heterogeneous microarray format, which allows for the simultaneous detection of multiple threat agents, and cationic conjugated polymers (CCPs), which serve either to enhance microarray systems (both commercially available and custom-printed) by increasing signal output of a reporter dye via Forster resonance energy transfer (FRET) or to provide a means for reporting in label-free microarray systems that could greatly reduce the cost for time and materials. For labeled systems, amplified signal intensities of reporting dyes should translate to either a higher sensitivity or lower requirements for optical specifications. Simplification of the optics would not only reduce the overall cost of testing, but opens the door for the overriding goal of design and development of portable and/or point-of-care testing devices. These types of diagnostic devices would have the potential to perform field-deployable, simple, and cost-effective testing in a timely fashion for bio-threat agents.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2006
Accession Number
ADA481619

Entities

People

  • Alexander Mikhailovsky
  • Brent Gaylord
  • Chengjun Sun
  • Chunyan Chi
  • Guillermo C. Bazan
  • Janice Hong
  • Jennifer Horsmon
  • Jennifer W Sekowski
  • Tsu-ju Fu

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Chemistry
  • Conjugated Polymers
  • Detection
  • Detectors
  • Dna Microarrays
  • Energy Transfer
  • Intensity
  • Light Sources
  • Materials
  • Molecules
  • Nucleic Acids
  • Point-Of-Care Diagnostic Testing
  • Polymers
  • Sensitivity
  • Standards
  • Staphylococcus Aureus

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Integrated Circuit Design and Technology.
  • Systems Analysis and Design