Design and Engineering of a Multi-Target (Multiplex) DNA Simulant to Evaluate Nulceic Acid Based Assays for Detection of Biological Threat Agents

Abstract

We designed and engineered a non-infectious Bio-threat simulant that included the nucleic acid signature of Bacillus anthracis, Yersinia pestis, Coxiella Burneti, Brucella sp., Francicella tularensis, Entherohemorragic E. coli, O157:H7, Burkholderia mallei, Burkholderia pseudomallei and Variola virus (smallpox virus). A chimera of 2040 bp was engineered to produce PCR amplicons of different sizes in a single Multiplex reaction designed for the rapid identification of the threat agents selected above.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2006
Accession Number
ADA481840

Entities

People

  • Jose-luis Sagripanti
  • Monica Carrera

Organizations

  • Edgewood Chemical Biological Center

Tags

DTIC Thesaurus Topics

  • Acids
  • Animals
  • Bacteria
  • Detection
  • Detectors
  • Diseases And Disorders
  • Engineering
  • Escherichia Coli
  • Infectious Diseases
  • Lepidoptera
  • Microorganisms
  • Molecules
  • Nucleic Acids
  • Q Fever
  • Spreadsheet Software
  • Viruses
  • Warning Systems

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Biotechnology