Fluoride Ion Regeneration of Cyclosarin (GF) From Minipig Tissue and Fluids Following Whole Body GF Vapor Exposure
Abstract
Recent developments to improve nerve agent biomarker techniques include methods for measuring fluoride regenerated Sarin (GB) in blood and tissue. Our efforts extend the fluoride ion regeneration method to be able to determine cyclosarin (GF) in red blood cells, plasma, and tissue of minipig blood samples after whole body exposure to GF at miosis levels. Blood samples were taken serially before, during, and after whole body GF exposure from the minipig via venous catheter allowing agent exposure profiles to be generated. After processing the samples with fluoride ion and extracting with C-18 solid phase extraction cartridges the ethyl acetate extract was analyzed by GC/MS. The GC/MS method utilized an autoinjector, a large volume injector port (LVI), positive ion ammonia chemical ionization detection in the SIM mode, and a 2H11-GF stable isotope internal standard. Results indicated that the method range was 10-1000 pg on column. The detection limit was 3 pg of GF on column despite the complexity of the red blood cell/tissue matrix. Conditions that needed to be optimized for the LVI included injection volume, initial temperature, pressure, and flow rate. The regenerated GF (R-GF) profiles differ greatly from the regenerated GB (R-GB) profiles in the minipig at similar exposure levels. The onset of the appearance of R-GF in the blood seems to be delayed and maximum levels are reached at much later times as compared to GB exposures. The rate of R-GB production was 5- 10 times greater than that of R-GF at equimolar exposures.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2006
- Accession Number
- ADA481857
Entities
People
- Edward M. Jakubowski
- Jeffery M. Mcguire
- Robert J. Mioduszewski
- Ronald A. Evans
- Sandra A. Thomson
- Stanley W. Hulet
Organizations
- Edgewood Chemical Biological Center