Synthetic Biomimetic Fluorophores for Micro/Nanosensor

Abstract

Proteins common in nature provide a rich source of potential fluorophores that can be used as taggent materials. Many of these fluorophores have been demonstrated to be effective molecular probes (e.g., green fluorescent protein (GFP)). The objective of this research is to find, isolate, sequence, and synthetically produce (from a natural source) synthetic fluorophores extracted from reef coral or rcGFP. From this investigation we discovered two novel clones with unique sequences for rcGFP and rcCFP. This research provided a better understanding of rcGFP's suitability for incorporation into micro or nano-sensory devices. Once synthetically created, these fluorophores are meant for design as triggering and signaling devices. These optical switches will possess far greater environmental stability and survivability than their natural analogs. Micro and nano-sensory devices are envisioned as helping the soldier to better understand the battlespace environment by providing remotely sensed, geospatial awareness.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2006
Accession Number
ADA481868

Entities

People

  • C. B. Smith
  • J. E. Anderson
  • P. M. Gillevet

Organizations

  • Engineer Research and Development Center

Tags

Communities of Interest

  • Weapons Technologies

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Biochemistry
  • Blood
  • Cells
  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Cnidarians
  • Deoxyribonucleic Acids
  • Fluorescence
  • Fluorophores
  • Genetics
  • Luminescence
  • Nanosensors
  • Polymerase Chain Reaction
  • Thermal Cyclers

Readers

  • Molecular Genetics
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology