The Role of ATF3, a New p53 Regulator, in Prostate Carcinogenesis
Abstract
ATF3 is a newly-identified p53 regulator, whose expression is frequently down-regulated in prostate cancers. The overall goal of this project is to elucidate the role of ATF3 in prostate carcinogenesis. Towards this aim, we are in a process of determining whether ATF3-deficient mice are prone to prostate carcinogenesis (Specific Aim #1) as well as whether ATF3 deficiency promotes survival of prostate epithelia cells (Specific Aim #2). Our major task in this report period (~6 months)is to establish a colony of ATF3 knockout (KO) mice in our animal facility and allow it to expand for subsequent studies. We have successfully completed this task, and obtained ~30 KO mice - 10 of them will be housed until moribund to determine whether aged mice grow prostate lesions (Task #1) while others will be bred with WT mice and ARR2Pbi-Cre/Pten(-/-) mice for carcinogenesis studies. In addition, we have developed several ATF3-knockdown LNCaP cell lines using a retroviral-based shRNA technology. These cells will be used to determine the effects of ATF3 on the survival of prostate cancer cells (Task #4).
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2008
- Accession Number
- ADA482293
Entities
People
- Chunhong Yan
Organizations
- Albany Medical College