Deposition of Selected Airborne Particles into a Microfluidic Flow Cytometer for Bioanalysis

Abstract

In a proof-of-concept experiment, we demonstrate how selected airborne bacteria, detected in air by light scattering, can be deflected aerodynamically into the input well of a microfluidic flow cytometer and analyzed using fluorescein-labeled antibodies. This experiment demonstrates a new method for introducing airborne samples into a microfluidic cell. The results suggest that by deflecting only those airborne particles having the fluorescence spectra of bioaerosols and by depositing the individual bioaerosols into the smallest volume of reagent required for analysis, it should be possible to achieve a system that can continuously monitor the air for certain bioaerosols while minimizing reagent usage.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2008
Accession Number
ADA482360

Entities

People

  • Hermes Huang
  • Richard K. Chang
  • Steven C. Hill
  • Yong-Le Pan

Organizations

  • United States Army Research Laboratory

Tags

Communities of Interest

  • Energy and Power Technologies
  • Sensors

DTIC Thesaurus Topics

  • Aerosol Generators
  • Aerosols
  • Bacteria
  • Biological Aerosols
  • Detection
  • Detectors
  • Fluorescence
  • Fungi
  • Laser Induced Fluorescence
  • Laser-Induced Breakdown Spectroscopy
  • Lasers
  • Light Scattering
  • Military Research
  • Optical Properties
  • Particles
  • Scattering
  • Spectra

Readers

  • Aerosol Science/Aerosol Physics
  • Nanocomposite Materials Science
  • Optical Physics and Photonics.