New Materials Based on Spider Silk

Abstract

In this project, we investigated the dragline silk proteins ADF-3 and ADF-4 of the spider Araneus diadematus. Adf-3 and Adf-4 cDNA can each be expressed directly in insect cells using the Baculovirus-expression-System. Recombinant ADF-4 is insolubly produced in the cytoplasm of insect cells and soluble produced upon secretion into the media. Further, parameters influencing stability and solubility of recombinantly produced ADF analogues have been investigated. We detected that intermolecular disulfide bridges formed between monomers of the ADF analogues stabilized formed dimer. Additionally, it was found that potassium phosphate triggers assembly of ADF analogues into spheres and fibrils.

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Document Details

Document Type
Technical Report
Publication Date
Nov 06, 2007
Accession Number
ADA482569

Entities

People

  • C. Vendrely
  • T. Scheibel

Organizations

  • Technical University of Munich

Tags

Communities of Interest

  • Engineered Resilient Systems

DTIC Thesaurus Topics

  • Abstracts
  • Cell Line
  • Cells
  • Culture Techniques
  • Department Of Defense
  • Electron Microscopy
  • Engineering
  • Escherichia Coli
  • Genetic Structures
  • Insects
  • Lepidoptera
  • Materials
  • Mathematics
  • Medical Personnel
  • Production
  • Scanning Electron Microscopy
  • Spiders

Readers

  • Energy Conservation and Renewable Energy Engineering.
  • Molecular and Cellular Biochemistry
  • Nanoscale Plasmonic Nanotechnology