Genetic Screening of Cells with Enhanced Antibody Production

Abstract

Here we report the progress made for grant No. W911NF-05-C-0059 between the entire funding period, 6/1/05 and 8/31/06. We have completed the study proposed: to use Functional Genetics' proprietary technology, Randon Homozygous Knock Out (RHKO) to increase antibody production from a production cell line, in this case CHO cells, as measured by Specific Productivity Rate (SPR). We also identified 2 candidate RHKO genes that cause increased SPR. Finally, we report the validation of one of these two genes.

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Document Details

Document Type
Technical Report
Publication Date
Jan 22, 2007
Accession Number
ADA482585

Entities

People

  • Roxanne Duan

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Antibodies
  • Base Lines
  • Cell Line
  • Cells
  • Culture Techniques
  • Fluorescence
  • Genes
  • Genetics
  • Industrial Production
  • Low Temperature
  • Phenotypes
  • Production
  • Productivity
  • Proteins
  • Secretion
  • Sequences

Fields of Study

  • Biology

Readers

  • Linear Algebra
  • Molecular Biology and Genetics
  • Regression Analysis.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech