Structure-Based Design of Inhibitors to the Cytotoxin RICIN. Addendum

Abstract

Ricin is a cytotoxin and a known bioterrorist weapon. The Army is pursuing anti-ricin vaccines, but plans to develop an efficacious antidote to the toxin for cases where vaccination is not appropriate. The goal of this project was to use the X-ray structure of ricin. A chain (RTA) as a template for inhibitor design. Computer modeling and X-ray screening aid in the design process. inhibitors which bind to the RTA substrate specificity site have been identified. Several chemical platforms including 9-oxo-guanine and 9-deazaguanine have been shown to bind in the RTA active site and act as a weak inhibitors. However efforts to derivatize and diversify the platforms via several straties including triazole "click" chemistry have met with unanticipated difficulties. None of the new compounds exhibits greatly improved inhibitory properties. A virtual screen of available compounds suggests pyrimidines may provide a useful platform for future work.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2008
Accession Number
ADA482673

Entities

People

  • Jon Robertus

Organizations

  • University of Texas at Austin

Tags

Communities of Interest

  • Weapons Technologies

DTIC Thesaurus Topics

  • Algorithms
  • Antidotes
  • Chemical Synthesis
  • Chemistry
  • Computer Programs
  • Computers
  • Crystallography
  • Cytotoxins
  • Databases
  • Inhibitors
  • Organic Chemistry
  • Platforms
  • Small Molecules
  • Substrates
  • Three Dimensional
  • X Rays
  • X-Ray Crystallography

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology