Effects of Radiation on Proteasome Function in Prostate Cancer Cells

Abstract

The proposed research in this proposal is based on our observation that the 26S proteasome is a direct target of radiation. In our initial statement of work, we proposed experiments investigating association and release of PIPs from the 26S proteasome as well as to study structural changes of the proteasome in response to radiation. We further intended to clarify to which extend proteasome subunit expression is regulated at the transcriptional, and posttranslational level in response to ionizing radiation. The final goal is to investigate these changes using cutting edge proteomic tools to identify the radiation target within the proteasome in prostate cancer. The aims are unaltered from the original submission.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2008
Accession Number
ADA482676

Entities

People

  • Frank Pajonk

Organizations

  • University of California, Los Angeles

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Electron Microscopy
  • Electrospray Ionization
  • Gel Electrophoresis
  • Inhibition
  • Ionization
  • Ionizing Radiation
  • Liquid Chromatography
  • Mass Spectra
  • Mass Spectrometry
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Proteins
  • Proteomics
  • Radiation
  • Spectra

Fields of Study

  • Physics

Readers

  • Molecular Biology and Genetics
  • Nuclear and Radiation Engineering.
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech