Development of Antigen Presenting Cells for Adoptive Immunotherapy in Prostate Cancer

Abstract

While adoptive immunotherapy holds promise as a treatment for cancer and infectious diseases, development has been impeded by the lack of reproducible methods for generating therapeutic numbers of antigen-specific CD8+ CTL. As a result, there are only limited reports of expansion of antigen-specific CTL to levels required for clinical therapy. Therefore, our groups has previously developed artificial Antigen-Presenting Cells (aAPC), made by coupling soluble HLAIg and anti-CD28 to beads. These aAPC have successfully been used to induce and expand CTL specific for CMV or melanoma. For the current study we have proposed to used and further developed those aAPC for the generation of prostate cancer specific CTL. Our preliminary data demonstrate that aAPC loaded with the prostate cancer specific antigen EpHA2 have been used to generate functional active prostate cancer-specific CTL from peripheral blood healthy donors.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2007
Accession Number
ADA482909

Entities

People

  • Mathias Oelke

Organizations

  • Johns Hopkins University

Tags

DTIC Thesaurus Topics

  • Biological Staining And Labeling
  • Biomedical Research
  • Cell Line
  • Cells
  • Couplings
  • Demographic Cohorts
  • Department Of Defense
  • Diseases And Disorders
  • Frequency
  • Immunotherapy
  • Infectious Diseases
  • Lymphocytes
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Standards
  • Tumor Cell Line

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech