A Novel Approach to the Development of Highly Specific Inhibitors of EFG, a Critical Transcription Factor in Prostate Cancer
Abstract
We have created a series of six constructs to express varying length fragments of ERG. Expression and purification protocols have been established for all six fragments. Using fluorescein-labeled oligonucleotides, we have developed a fluorescence polarization based assay for measuring the DNA binding of the ERG fragments to a functional DNA element. Using this assay, we have shown that longer fragments of ERG show decreased DNA binding, i.e. are auto-inhibited. Based on this data, we have focused efforts on producing one fragment in the labeled form necessary for NMR structural studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2008
- Accession Number
- ADA482921
Entities
People
- John H. Bushweller
- Tomasz K. Kabzinski
Organizations
- University of Virginia