Characterization of Mustard-Stimulated Protease by Zymography

Abstract

Protease stimulation in human epidermal keratinocytes (HEK) due to mustard exposure is well established. However, the specific protease(s) stimulated by mustard remain to be determined. We used zymography technique to characterize the mustard-stimulated protease in HEK extracts. The major proteolytic band in 70kDa molecular weight was observed in mustard-exposed cells. Addition of a serine protease inhibitor (ICD 2812) or EGTA decreased this band. The protease from mustard treated HEK was electro eluted from the zymogram gel and was found to hydrolyze the synthetic substrate Chromozyme TRY, supporting that it could be a calcium dependent serine-protease.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2003
Accession Number
ADA483522

Entities

People

  • Cynthia Rosenthal
  • Dean Rosenthal
  • Prabhati Ray
  • Radharaman Ray
  • Xiannu Jin
  • Yen Leng
  • Zhuangwu Li

Organizations

  • Walter Reed Army Institute of Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Biochemistry
  • Biology
  • Biomedical Research
  • Cells
  • Enzyme Inhibitors
  • Epithelial Cells
  • Information Operations
  • Inhibitors
  • Instructions
  • Molecular Biology
  • Molecular Weight
  • Nitrogen Mustards
  • Proteins
  • Skin
  • Standards
  • Therapy

Fields of Study

  • Biology

Readers

  • Geochemistry
  • Molecular and Cellular Biochemistry