Real-Time Fluorogenic Polymerase Chain Reaction (PCR) Assays to Nucleic Acid-Based Detection of Simulants and Biothreat Agents

Abstract

Polymerase chain reaction (PCR) is an exquisitely sensitive method for the amplification and detection of genetic material (DNA and RNA sequences). We have developed several assays using a variation of PCR that proceeds very quickly and allows the monitoring of the progress of the reaction in real time. We describe here assays for the detection of the gene encoding staphylococcal enterotoxin A (SEA), a toxin produced by the bacterium Staphylococcus aureus and an agent responsible for a significant fraction of food poisoning incidents worldwide. We also describe several assays for the detection of a simulant of viral pathogens, the bacteriophage MS2.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2003
Accession Number
ADA483954

Entities

People

  • Akbar S. Khan
  • Cheng J. Cao
  • James J. Valdes
  • Jennifer R. Bucher
  • Kevin P. O'connell
  • Mark V. Gostomski
  • Patricia E. Anderson

Tags

DTIC Thesaurus Topics

  • Acids
  • Bacteriophages
  • Chain Reactions
  • Chemical Reactions
  • Chemistry
  • Detection
  • Detectors
  • Dyes
  • Fluorescence
  • Fluorescent Dyes
  • Leviviridae
  • Microbial Genome
  • Molecules
  • Nucleic Acids
  • Pathogenic Bacteria
  • Polymerase Chain Reaction
  • Standards

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular and Cellular Biochemistry
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology