Fluorescence Hybridization Assay Based On Chitosan-Linked Softarrays
Abstract
The work to be presented is examining the development of a hybridization-based biosensor. The design of a high throughput assay based on standard laboratory instrumentation and 96-well microtiter plates will be discussed as well as a highly sensitive sensor using a novel tapered fiber optic probe. The immobilization chemistry learned in the 96-well plate will be implemented on the fiber optic sensor. The high throughput assay is based on the immobilization of single-stranded, synthetic oligonucletides inside a 96-well plate utilizing chitosan and glutaraldehyde. Chitosan is a biopolymer with abundant primary amines for attachment chemistry and is easily deposited as thin layers on surfaces. Hybridization can take place inside the wells with a complementary analyte sequence followed by a fluorescent, labeled signaling probe to form a sandwich assay. The hybridized wells can be interrogated with any bench-top fluorometer with a microwell plate reader.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA484090
Entities
People
- Gregory F Payne
- Hyunmin Yi
- James B. Gillespie
- James J. Sumner
- Li-qun Wu
- William E. Bentley
Organizations
- United States Army Research Laboratory