Molecular Aspects of Muscle Damage and Denervation with Public Access Tools
Abstract
Two Tasks were originally proposed; one was design, coding and implementation of PEPR (Public expression profiling resource). This was accomplished, and PEPR has become one of the most heavily used mRNA expression profiling (microarray) resources worldwide. We have populated with 7,000 microarray profiles, of which many are related to nerve and muscle damage and repair, and are of high relevance to health of the military. Of these, approximately 3,000 microarray profiles are in the public domain arm of PEPR, and have been co-submitted to NIH NCBI GEO through an automated data submission pipeline developed under the auspices of this award. Downloads of microarray data by the international research community is provided at no charge, and currently averages about 6,000 array downloads per month. This has effectively parallelized research on issues of importance the health of military recruits, including brain trauma, spinal cord injury, muscle exercise, muscle damage, and regenerative science. We also designed, coded and implemented HCE (Hierarchical Clustering Explorer). This powerful public domain software package has been downloaded by thousands of investigators, and facilitated thousands of research studies. Task 2 was focused on the proteomics of muscle atrophy and repair. Through support of the DoD award, we established proteomics expertise in the Research Center for Genetic Medicine. We also increased knowledge of the molecular pathways in muscle and nerve, damage. 21 publications in peer reviewed journals supported in whole or in part by the 2 yr DoD award. Some of these have been cited by the journals as "most highly accessed" of papers published.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2005
- Accession Number
- ADA484106
Entities
People
- Eric Hoffman