Impact of Erb-B Signaling on Myelin Repair in the CNS Following Virus-Induced Damage
Abstract
The objective of these studies is to examine the impact of erbB-mediated signaling on myelin destruction and repair in a novel murine model of central nervous system (CNS) demyelination. In these studies, we utilize a model of demyelination triggered by direct injection of Theiler's murine encephalomyelitis virus (TMEV) into the spinal cord. This method of lesioning permits us to precisely identify the age and site of a lesion. We hypothesize that increased erbB-mediated signaling will protect animals from disease. Conversely, reduced signaling will worsen disease. Toward the goals of this project, we have generated three recombinant adenoviral constructs that will be used to exogenously increase the level of ligand available to the receptors, thus examining whether enhanced signaling via erbB family members will enhance protection of the CNS. We have identified the major cell type harboring virus in this model (macrophages) and have begun to examine the effects of infection on myelin gene transcription.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2008
- Accession Number
- ADA484171
Entities
People
- Kristen M. Drescher
Organizations
- Creighton University