Fluoride Ion Regeneration of Cyclosarin (GF) From Rat Blood Following Whole-Body Exposure to Lethal Levels of GF Vapor
Abstract
A sensitive and selective method has been developed for a cyclohexyl methylphosphonofluoridate (GF) nerve agent biomarker in tissue and biological fluids after GF lethal level whole body inhalation exposure. Levels of exposure ranged from 2 mg/m3 for 240 min to 41.9 mg/m3 for 10 min. The GF biomarker found in rat plasma and red blood cell samples was regenerated GF, which is the product of adding fluoride ion at pH 4 to the post exposure samples. Regenerated GF was separated from the biological matrix using a C18 solid-phase extraction (SPE) sample preparation. Samples are concentrated by injecting 2-200 uL of ethyl acetate extract on a Tenax -TA sorbent tube along with 200 pg of decadeuterated diethyl ethyl phosphonate as the internal standard followed by thermal desorption GC-FPD analysis and GC-MS confirmation. The method detection limit was approximately 10 pg of agent on column. Quantities of regenerated GF found in plasma samples ranged from 230 to 572 ng/g. Red blood cell samples from the same animals contained considerably less biomarker ranging from 1.45 to 30.0 ng/g. In cases where animals died just after the exposure was complete and post mortem blood could be collected the levels of regenerated GF in both plasma and red blood cells were only 60 % of the amount found in the living rats.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA484392
Entities
People
- B. I. Gaviola
- C. E. Whalley
- D. C. Burnett
- E. M. Jakubowski
- J. A. Scotto
- J. H. Manthei
- J. S. Anthony
- R. A. Way
- R. J. Mioduszewski
- W. T. Muse
Organizations
- Edgewood Chemical Biological Center